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3SE5

Fic protein from NEISSERIA MENINGITIDIS mutant delta8 in complex with AMPPNP

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyCCD
Collection date2011-04-14
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1.0000
Spacegroup nameP 1 21 1
Unit cell lengths73.945, 65.031, 75.995
Unit cell angles90.00, 107.08, 90.00
Refinement procedure
Resolution15.000 - 1.700
R-factor0.16
Rwork0.159
R-free0.19700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2g03
RMSD bond length0.011
RMSD bond angle1.317
Data reduction softwareMOSFLM
Data scaling softwareSCALA (CCP4_3.3.16 2010/01/06)
Phasing softwarePHASER
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]37.03037.0301.790
High resolution limit [Å]1.7005.3801.700
Rmerge0.0690.0300.370
Total number of observations983536210
Number of reflections73865
<I/σ(I)>9.814420.861.97
Completeness [%]97.798.2595.43
Redundancy44.033.46
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.629344% w/v PEG 600, 0.1M Na-citrate, pH 5.6, vapor diffusion, hanging drop, temperature 293K

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