3SDF
Crystal Structure of C-lobe of Bovine lactoferrin Complexed with Lipoteichoic acid at 2.1 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-05-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.417, 50.398, 65.944 |
| Unit cell angles | 90.00, 107.72, 90.00 |
Refinement procedure
| Resolution | 62.000 - 2.100 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.23441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rgy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.246 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.820 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 21244 | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 94.3 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01M Znso4, 0.1M MES, 25% PEG, Monomethyl Ether 550 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
