3SCV
Crystal Structure of Rice BGlu1 E386G/S334A Mutant Complexed with Cellotetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2011-03-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.433, 101.508, 127.317 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.760 - 2.110 |
R-factor | 0.17423 |
Rwork | 0.173 |
R-free | 0.20597 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgl |
RMSD bond length | 0.010 |
RMSD bond angle | 1.332 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.5.0110) |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 60078 | |
<I/σ(I)> | 13 | 4.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 16% PEG MME 5000, 0.18M ammonium sulfate, 0.1M MES, 0.002M cellotetraose, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K |