3SCT
Crystal Structure of Rice BGlu1 E386G Mutant Complexed with Cellotetraose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2009-01-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.270, 101.042, 127.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.670 - 1.600 |
R-factor | 0.19071 |
Rwork | 0.190 |
R-free | 0.20845 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rgl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.5.0110) |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 134069 | |
<I/σ(I)> | 19 | 3.5 |
Completeness [%] | 99.1 | 97.3 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 288 | 22% PEG MME 5000, 0.22M ammonium sulfate, 0.1M MES, 0.002M cellotetraose, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K |