3SC3
Crystal structure of a Putative DNA replication regulator Hda (Sama_1916) from SHEWANELLA AMAZONENSIS SB2B at 3.00 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-15 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | I 2 3 |
Unit cell lengths | 162.440, 162.440, 162.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.881 - 3.001 |
R-factor | 0.2267 |
Rwork | 0.224 |
R-free | 0.25320 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2007) |
Phasing software | SHELX |
Refinement software | PHENIX (1.4) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.881 | 3.170 | |
High resolution limit [Å] | 3.000 | 6.750 | 3.000 |
Rmerge | 0.143 | 0.014 | 0.014 |
Number of reflections | 14422 | 1324 | 2175 |
<I/σ(I)> | 10.68 | 27.2 | 1.6 |
Completeness [%] | 99.9 | 99.4 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.26M (NH4)2SO4, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |