3SBT

Crystal structure of a Aar2-Prp8 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9814
Spacegroup name	C 1 2 1
Unit cell lengths	89.334, 82.019, 94.057
Unit cell angles	90.00, 108.59, 90.00

Refinement procedure

Resolution	26.735 - 1.799
R-factor	0.1637
Rwork	0.162
R-free	0.19670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.197
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_353))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.100	1.830
High resolution limit [Å]	1.799	1.800
Number of reflections	59439
Completeness [%]	99.6	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M Tris-HCl, pH 8.5, 0.1 M lithium sulfate, 20 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K