3SBG
Crystal structure of a Prp8 C-terminal fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9814 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.786, 73.598, 72.882 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 27.077 - 3.280 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.420 |
| High resolution limit [Å] | 3.280 | 3.300 |
| Number of reflections | 11830 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1 M ammonium tartrate, 5 mM NiCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
