3SBG
Crystal structure of a Prp8 C-terminal fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9814 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.786, 73.598, 72.882 |
Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
Resolution | 27.077 - 3.280 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.623 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.420 |
High resolution limit [Å] | 3.280 | 3.300 |
Number of reflections | 11830 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 1 M ammonium tartrate, 5 mM NiCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |