3SBD

Crystal structure of RAC1 P29S mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-27
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.266, 80.021, 94.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.680 - 2.100
R-factor	0.24007
Rwork	0.238
R-free	0.28460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mh1
RMSD bond length	0.014
RMSD bond angle	1.576
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	23040
<I/σ(I)>	10.1	1.5
Redundancy	5.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	20% PEG4000, 7% isopropanol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K