3SB7
Cu-mediated Trimer of T4 Lysozyme D61H/K65H/R76H/R80H by Synthetic Symmetrization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | H 3 |
Unit cell lengths | 119.910, 119.910, 64.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.472 - 2.700 |
R-factor | 0.1885 |
Rwork | 0.187 |
R-free | 0.22610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.572 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 27.472 | 2.770 | |
High resolution limit [Å] | 2.700 | 12.070 | 2.700 |
Rmerge | 0.113 | 0.049 | 0.508 |
Number of reflections | 9419 | 92 | 685 |
<I/σ(I)> | 9.75 | 21.33 | 3.29 |
Completeness [%] | 98.9 | 86.8 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2M Potassium Thiocyanate, 25% PEG 3350, vapor diffusion, hanging drop, temperature 290K |