3S8D
Crystal Structure of RipA from Yersinia pestis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-07-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.533, 109.306, 85.303 |
| Unit cell angles | 90.00, 120.20, 90.00 |
Refinement procedure
| Resolution | 26.701 - 2.310 |
| R-factor | 0.2044 |
| Rwork | 0.201 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.1_741) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 38932 |
| Completeness [%] | 94.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | 18% PEG 3350, 0.2M sodium citrate, 0.1M Bis-tris propane, 1mM acetyl CoA, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
