3S8C

Structure of Yeast Ribonucleotide Reductase 1 R293A with AMPPNP and CDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Collection date	2011-03-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	107.781, 116.810, 64.126
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.290 - 2.770
R-factor	0.20236
Rwork	0.196
R-free	0.25588
Structure solution method	Isomorphous
RMSD bond length	0.017
RMSD bond angle	1.748
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.290	2.850
High resolution limit [Å]	2.770	2.770
Rmerge	0.140	0.580
Number of reflections	18704
<I/σ(I)>	15.2	2.2
Completeness [%]	98.3	99.6
Redundancy	7.2	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	298	20-25% PEG 3350, 0.1 M HEPES, 0.1 M sodium chloride, pH 7.0, EVAPORATION, temperature 298K