3S8B
Structure of Yeast Ribonucleotide Reductase 1 with AMPPNP and CDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.869, 117.567, 64.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.030 - 2.800 |
R-factor | 0.21289 |
Rwork | 0.209 |
R-free | 0.25027 |
Structure solution method | Isomorphous |
RMSD bond length | 0.010 |
RMSD bond angle | 1.270 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.030 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.130 | 0.520 |
Number of reflections | 18651 | |
<I/σ(I)> | 12.5 | 2.8 |
Completeness [%] | 98.8 | 97 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 298 | 20-25% PEG 3350, 0.1 M HEPES, 0.1 M sodium chloride, pH 7.0, EVAPORATION, temperature 298K |