3S8A
Structure of Yeast Ribonucleotide Reductase R293A with dGTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-02 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 108.443, 117.716, 64.781 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.990 - 2.900 |
R-factor | 0.23485 |
Rwork | 0.228 |
R-free | 0.29965 |
Structure solution method | Isomorphous |
RMSD bond length | 0.014 |
RMSD bond angle | 1.643 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.970 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.130 | 0.542 |
Number of reflections | 16450 | |
<I/σ(I)> | 10.1 | 2.2 |
Completeness [%] | 96.5 | 98 |
Redundancy | 4.14 | 4.35 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 298 | 20-25% PEG 3350, 0.1 M HEPES, 0.1 M sodium chloride, pH 7.0, EVAPORATION, temperature 298K |