3S87
Structure of Yeast Ribonucleotide Reductase 1 with dGTP and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-02 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.618, 117.191, 65.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.2099 |
| Rwork | 0.204 |
| R-free | 0.26062 |
| Structure solution method | Isomorphous |
| Starting model (for MR) | 2cvx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.072 | 0.585 |
| Number of reflections | 35900 | |
| <I/σ(I)> | 15 | 2.07 |
| Completeness [%] | 99.8 | 95.4 |
| Redundancy | 5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7 | 298 | 20-25% PEG 3350, 0.1 M HEPES, 0.1 M sodium chloride, pH 7.0, EVAPORATION, temperature 298K |
