3S7T
Crystal structure of SeMet B. licheniformis CDPS YvmC-Blic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.172, 54.713, 101.556 |
| Unit cell angles | 90.00, 130.05, 90.00 |
Refinement procedure
| Resolution | 38.871 - 2.808 |
| R-factor | 0.187 |
| Rwork | 0.182 |
| R-free | 0.23240 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.237 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
| Rmerge | 0.148 | 0.076 | 0.401 |
| Number of reflections | 15530 | ||
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 13.5 | 13.3 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | PEG 10000 14%, NaCl 100mM, 20% glycerol, pH 8.5, vapor diffusion, hanging drop, temperature 293K |
