3S7R
Crystal structure of a Heterogeneous nuclear ribonucleoprotein A/B (HNRPAB) from HOMO SAPIENS at 2.15 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-10 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91162,0.97934,0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.150, 41.856, 55.146 |
Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
Resolution | 30.028 - 2.150 |
R-factor | 0.1793 |
Rwork | 0.177 |
R-free | 0.22530 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XSCALE (December 6, 2010) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.028 | 30.028 | 2.230 |
High resolution limit [Å] | 2.150 | 4.610 | 2.150 |
Rmerge | 0.086 | 0.034 | 0.571 |
Number of reflections | 10785 | 2061 | 2151 |
<I/σ(I)> | 7.05 | 18.6 | 1.6 |
Completeness [%] | 97.3 | 97.2 | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 40.00% polyethylene glycol 600, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |