3S7Q
Crystal Structure of a Monomeric Infrared Fluorescent Deinococcus radiodurans Bacteriophytochrome chromophore binding domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9785 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 95.129, 55.072, 69.969 |
Unit cell angles | 90.00, 92.23, 90.00 |
Refinement procedure
Resolution | 27.650 - 1.748 |
R-factor | 0.18881 |
Rwork | 0.187 |
R-free | 0.21693 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o9c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.552 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.790 |
High resolution limit [Å] | 1.748 | 1.748 |
Number of reflections | 36431 | |
<I/σ(I)> | 22.1 | 4.7 |
Completeness [%] | 99.2 | 100 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 3% v/v PEG 1000, 20% v/v ethanol, 6% v/v glycerol, 0.1M phosphate citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |