3S7G
Aglycosylated human igg1 fc fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-25 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 65.689, 65.773, 77.811 |
Unit cell angles | 98.18, 100.50, 116.92 |
Refinement procedure
Resolution | 45.000 - 3.130 |
R-factor | 0.262 |
Rwork | 0.259 |
R-free | 0.32300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3DNK (CH2 AND CH3 DOMAINS USED SEPARATELY) |
RMSD bond length | 0.017 |
RMSD bond angle | 1.633 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.130 | 3.130 |
Rmerge | 0.061 | 0.347 |
Number of reflections | 18883 | |
<I/σ(I)> | 12.6 | 2.8 |
Completeness [%] | 97.1 | |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 10% PEG 1000, 20% PEG 6000, 0.1M MAGNESIUM CHLORIDE, 0.2M TRIS, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 295K |