3S7C

Crystal structure of a 2'-azido-uridine-modified RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	P 43
Unit cell lengths	29.610, 29.610, 76.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.610 - 1.100
R-factor	0.118
Rwork	0.117
R-free	0.15590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.005
RMSD bond angle	0.969
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.130
High resolution limit [Å]	1.100	1.100
Rmerge		0.454
Number of reflections	26490
<I/σ(I)>		2.91
Completeness [%]	99.6	94.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1 volume of [3.1M (NH4)2SO4, 50mM MOPS pH7.0, 10mM MgCl2, 10mM MnCl2] + 2 volumes of [RNA in 1mM NaEDTA, 10mM Tris pH8.0], VAPOR DIFFUSION, HANGING DROP, temperature 293K