3S6P

Crystal Structure of Helicoverpa Armigera Stunt Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	298
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	404.120, 405.570, 406.040
Unit cell angles	119.20, 114.44, 94.79

Refinement procedure

Resolution	40.000 - 2.500
R-factor	0.24
Rwork	0.237
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohf
RMSD bond length	0.011
RMSD bond angle	1.619
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.148	0.092	0.332
Number of reflections	1807513
<I/σ(I)>	6.5		2.1
Completeness [%]	27.3	45.7	12.1
Redundancy	1.2	1.2	1.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	2 ul of 8mg/ml virus solution (50mM NaAce pH 5.0) added to 2 ul of 100mM bicine pH 8.0, 1.0% PEG8000, 150mM CaAce, 350-400mM NaCl. Trapezoid crystals appear in 2 days and grow for 3 weeks, VAPOR DIFFUSION, SITTING DROP, temperature 295K
1	VAPOR DIFFUSION, SITTING DROP	8	295	2 ul of 8mg/ml virus solution (50mM NaAce pH 5.0) added to 2 ul of 100mM bicine pH 8.0, 1.0% PEG8000, 150mM CaAce, 350-400mM NaCl. Trapezoid crystals appear in 2 days and grow for 3 weeks, VAPOR DIFFUSION, SITTING DROP, temperature 295K