3S6E

Crystal structure of a RNA binding motif protein 39 (RBM39) from Mus musculuS at 0.95 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.95369,0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	44.701, 55.139, 84.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.200 - 0.950
R-factor	0.1197
Rwork	0.119
R-free	0.13150
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.832
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.200	28.200	0.970
High resolution limit [Å]	0.950	4.250	0.950
Rmerge		0.047	0.670
Total number of observations		11798	38502
Number of reflections	131023
<I/σ(I)>	14	60.7	1.9
Completeness [%]	99.4	99.5	96.7
Redundancy	5.2	7.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	20.00% polyethylene glycol 6000, 0.1M sodium citrate pH 5.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K