3S6C
Structure of human CD1e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9395 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.289, 45.930, 65.628 |
| Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
| Resolution | 51.550 - 2.900 |
| R-factor | 0.24332 |
| Rwork | 0.241 |
| R-free | 0.29551 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.832 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.110 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.063 | 0.372 |
| Number of reflections | 12460 | |
| <I/σ(I)> | 7 | 1.7 |
| Completeness [%] | 94.7 | 96.7 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 293 | 10% PEG 6000, 0.2 M Sodium malonate, 0.1 M magnesium valerate, 0.1 M sodium borate , pH 3.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
