3S63
Saposin-like protein Na-SLP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.94723 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 85.433, 85.433, 113.452 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.662 - 2.700 |
| R-factor | 0.1874 |
| Rwork | 0.184 |
| R-free | 0.24490 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 7191 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 41.7 | 43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2M NaCl, 20% PEG 6000, 0.1M HEPES, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
