3S5N
Crystal Structure of Human 4-hydroxy-2-oxoglutarate Aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 142.143, 142.143, 108.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.524 - 2.500 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.24580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.433 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER (2.1.1) |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.380 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.267 | |
| Number of reflections | 22814 | |
| <I/σ(I)> | 28.3 | 11.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 41.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 3350, KSCN, Bis tris Propane, TCEP, Ethelyene glycol, pH 8, vapor diffusion, hanging drop, temperature 298K |
