3S5F
Crystal structure of human frataxin variant W155F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-07 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.680, 53.120, 48.480 |
Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
Resolution | 44.850 - 1.500 |
R-factor | 0.166 |
Rwork | 0.164 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ekg |
RMSD bond length | 0.022 |
RMSD bond angle | 1.926 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.854 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 35399 | |
<I/σ(I)> | 13.8 | 2.2 |
Completeness [%] | 97.7 | 97.2 |
Redundancy | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2 M sodium acetate trihydrate, 0.1 M Tris hydrochloride, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |