3S5F
Crystal structure of human frataxin variant W155F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-07 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.680, 53.120, 48.480 |
| Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
| Resolution | 44.850 - 1.500 |
| R-factor | 0.166 |
| Rwork | 0.164 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ekg |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.926 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.854 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 35399 | |
| <I/σ(I)> | 13.8 | 2.2 |
| Completeness [%] | 97.7 | 97.2 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.2 M sodium acetate trihydrate, 0.1 M Tris hydrochloride, 30% PEG4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
