3S5E
Crystal structure of human frataxin variant W155R, one of the Friedreich's ataxia point mutations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-29 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.895, 49.215, 67.125 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.690 - 1.310 |
R-factor | 0.1531 |
Rwork | 0.151 |
R-free | 0.19690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ekg |
RMSD bond length | 0.023 |
RMSD bond angle | 1.822 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.690 | 1.380 |
High resolution limit [Å] | 1.310 | 1.310 |
Number of reflections | 31647 | |
<I/σ(I)> | 22.2 | 5.6 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 15 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 16% PEG2000 MME, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |