3S5E

Crystal structure of human frataxin variant W155R, one of the Friedreich's ataxia point mutations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-29
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	38.895, 49.215, 67.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.690 - 1.310
R-factor	0.1531
Rwork	0.151
R-free	0.19690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ekg
RMSD bond length	0.023
RMSD bond angle	1.822
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.690	1.380
High resolution limit [Å]	1.310	1.310
Number of reflections	31647
<I/σ(I)>	22.2	5.6
Completeness [%]	99.8	99.7
Redundancy	15	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	16% PEG2000 MME, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K