3S5D
Crystal structure of human frataxin variant W155A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.490, 32.260, 44.810 |
| Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
| Resolution | 44.800 - 1.500 |
| R-factor | 0.1698 |
| Rwork | 0.169 |
| R-free | 0.19210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ekg |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.633 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.800 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 20101 | |
| <I/σ(I)> | 12 | 6.6 |
| Completeness [%] | 99.3 | 98.6 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 1.8 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
