3S4Z

Structure of a Y DNA-FANCI complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Detector technology	CCD
Collection date	2011-01-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	I 21 21 21
Unit cell lengths	235.200, 307.900, 375.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.000 - 7.800
R-factor	0.31023
Rwork	0.310
R-free	0.32431
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	2.035
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	7.800
Number of reflections	14728
Completeness [%]	93.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	50 mM Hepes-Na, 10 mM DTT, 2.5 mM EDTA, 9 to 11 % (w/v) PEG 3350, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K