3S4R
Crystal structure of vimentin coil1A/1B fragment with a stabilizing mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 65.329, 65.329, 377.339 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.520 - 2.452 |
R-factor | 0.2929 |
Rwork | 0.292 |
R-free | 0.31470 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.217 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXS |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 6.650 | 2.450 |
Rmerge | 0.026 | 0.420 | |
Number of reflections | 11209 | ||
<I/σ(I)> | 14.5 | 1.7 | |
Completeness [%] | 93.7 | 99.1 | 55.9 |
Redundancy | 10.9 | 10.2 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.1M Na cacodylate, MPD 20%, Mg acetate 0.45M, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.1M Na cacodylate, MPD 20%, Mg acetate 0.45M, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 0.1M Na cacodylate, MPD 20%, Mg acetate 0.45M, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |