3S4L

The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii

Replaces:  3M5F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-07
Detector	ADSC QUANTUM 315
Spacegroup name	P 41 21 2
Unit cell lengths	59.073, 59.073, 162.879
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.537 - 2.300
R-factor	0.2355
Rwork	0.234
R-free	0.27230
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.122
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.540	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.040	0.350
Number of reflections	13603
<I/σ(I)>	38.3	8.3
Completeness [%]	99.9	99.9
Redundancy	13.7	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	293	PEG 5000 MME 25%, (NH4)2 SO4 0.2M, SPG 0.1M, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K