3S4L
The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii
Replaces: 3M5FExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-07 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 59.073, 59.073, 162.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.537 - 2.300 |
R-factor | 0.2355 |
Rwork | 0.234 |
R-free | 0.27230 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.122 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.540 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.040 | 0.350 |
Number of reflections | 13603 | |
<I/σ(I)> | 38.3 | 8.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 13.7 | 14.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 293 | PEG 5000 MME 25%, (NH4)2 SO4 0.2M, SPG 0.1M, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |