3S4L
The CRISPR-associated Cas3 HD domain protein MJ0384 from Methanocaldococcus jannaschii
Replaces: 3M5FExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.073, 59.073, 162.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.537 - 2.300 |
| R-factor | 0.2355 |
| Rwork | 0.234 |
| R-free | 0.27230 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.122 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.540 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.350 |
| Number of reflections | 13603 | |
| <I/σ(I)> | 38.3 | 8.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 13.7 | 14.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 293 | PEG 5000 MME 25%, (NH4)2 SO4 0.2M, SPG 0.1M, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
