3S4G

Low Resolution Structure of STNV complexed with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-01
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.488
Spacegroup name	C 1 2 1
Unit cell lengths	313.146, 302.121, 183.385
Unit cell angles	90.00, 93.82, 90.00

Refinement procedure

Resolution	95.850 - 6.000
R-factor	0.38
R-free	0.43000
Structure solution method	FOURIER SYNTHESIS
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	140.000	6.320
High resolution limit [Å]	6.000	6.000
Rmerge	0.128
Number of reflections	24647
<I/σ(I)>	11.8
Completeness [%]	99.0	97
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	298	0.4 % PEG 6000, pH 6.2-7.0, 1 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K