3S4C
Lactose phosphorylase in complex with sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9780 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 85.450, 93.210, 106.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.820 - 2.400 |
R-factor | 0.1826 |
Rwork | 0.182 |
R-free | 0.21410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.820 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 33519 | |
Completeness [%] | 98.9 | 89.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6M ammonium sulfate, 0.1M Mes monohydrate pH6.5, 10% 1,4-dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 298K |