3S4B
Cellobiose phosphorylase from Cellulomonas uda in complex with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.417, 102.740, 98.305 |
Unit cell angles | 90.00, 96.44, 90.00 |
Refinement procedure
Resolution | 19.950 - 2.400 |
R-factor | 0.2337 |
Rwork | 0.231 |
R-free | 0.28150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.071 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 54077 | |
Completeness [%] | 81.6 | 81.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 3.4M sodium formate, 0.1M sodium acetate trihydrate, pH4.6, 20mM alpha-D-glucose 1-phosphate soaked with 5M sodium formate, 0.1M sodium acetate trihydrate, pH4.6, 50mM alpha-D-glucose 1-phosphate, 50mM glucose, VAPOR DIFFUSION, HANGING DROP, temperature 298K |