3S4A
Cellobiose phosphorylase from Cellulomonas uda in complex with cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9330 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.690, 195.560, 103.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.790 - 1.990 |
R-factor | 0.2025 |
Rwork | 0.200 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.388 |
Data reduction software | PROTEUM2 |
Data scaling software | PROTEUM2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.790 | 2.050 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 118758 | |
Completeness [%] | 98.8 | 90.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | 4 M sodium formate, 20 mM GP, soaked with 5 M sodium formate, 20 mM cellobios , pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |