3S49
RNA crystal structure with 2-Se-uridine modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Detector technology | CCD |
| Collection date | 2010-08-01 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 47.095, 47.095, 424.655 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.2173 |
| Rwork | 0.215 |
| R-free | 0.26977 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 246d |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.169 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.511 | 0.890 |
| Number of reflections | 8288 | |
| <I/σ(I)> | 22 | 3.3 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 18.8 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 10% MPD, 40 mM Na Cacodylate (pH 7.0), 12mM Spermine Tetra-HCl, 80 mM Potassium Chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
