3S47

Crystal structure of enolase superfamily member from Clostridium beijerincki complexed with Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 4 21 2
Unit cell lengths	111.259, 111.259, 139.803
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.757 - 1.700
R-factor	0.2455
Rwork	0.243
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.071
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.757
High resolution limit [Å]	1.700
Number of reflections	85180
Completeness [%]	88.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	45% MPD, 0.1M Bis-Tris, 0.2M ammonium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K