3S2U
Crystal structure of the Pseudomonas aeruginosa MurG:UDP-GlcNAc substrate complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 49.930, 49.930, 278.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.230 |
R-factor | 0.2139 |
Rwork | 0.209 |
R-free | 0.27780 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.190 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.200 |
Number of reflections | 15355 |
Completeness [%] | 84.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 18% PEG3350, 0.1M HEPES pH 7.0 and 10mM DTT, VAPOR DIFFUSION, temperature 293K |