3S2C
Structure of the thermostable GH51 alpha-L-arabinofuranosidase from Thermotoga petrophila RKU-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.950, 187.292, 180.636 |
| Unit cell angles | 90.00, 90.87, 90.00 |
Refinement procedure
| Resolution | 49.240 - 3.000 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pz3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.389 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 137481 | |
| Completeness [%] | 97.9 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
