3S26
Crystal Structure of Murine Siderocalin (Lipocalin 2, 24p3)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.890, 43.960, 101.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.530 - 1.800 |
R-factor | 0.20216 |
Rwork | 0.201 |
R-free | 0.22605 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.495 |
Data reduction software | d*TREK (9.9.3D) |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.890 | 42.890 | 1.860 |
High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
Rmerge | 0.046 | 0.030 | 0.426 |
Number of reflections | 18574 | ||
<I/σ(I)> | 14.7 | 43.2 | 3.5 |
Completeness [%] | 99.9 | 99.8 | 100 |
Redundancy | 6.87 | 6.39 | 7.09 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPES, 20% PEG 4000, 10% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |