3S1Y
AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA) in complex with a beta-lactamase inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.253, 69.696, 101.667 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| R-factor | 0.18214 |
| Rwork | 0.181 |
| R-free | 0.20241 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.194 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | MERLOT |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.101 | 0.632 |
| Number of reflections | 74960 | |
| <I/σ(I)> | 8.2 | 1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.2 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 18% PEG 3350, 10% 2-propanol, 100 mM Imidazole, microseeding, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
