3S1R

RNA Polymerase II Initiation Complex with a 5-nt 3'-deoxy RNA soaked with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	160.670, 221.320, 193.210
Unit cell angles	90.00, 98.31, 90.00

Refinement procedure

Resolution	29.970 - 3.200
R-factor	0.1793
Rwork	0.177
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.300
Data scaling software	XSCALE
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.973		3.290
High resolution limit [Å]	3.200	14.320	3.200
Rmerge	0.164	0.011	0.011
Number of reflections	108840	1129	7741
<I/σ(I)>	9.26	42.6	1.7
Completeness [%]	99.4	88.1	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM dioxane, 9-11% PEG 6.000, 15mM DTT, vapor diffusion, temperature 293K