3S1Q
RNA Polymerase II Initiation Complex with a 5-nt 3'-deoxy RNA soaked with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.940, 220.530, 193.720 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 29.666 - 3.300 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data scaling software | XSCALE |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.666 | 3.380 | |
| High resolution limit [Å] | 3.300 | 14.750 | 3.300 |
| Rmerge | 0.118 | 0.010 | 0.010 |
| Number of reflections | 100159 | 1012 | 7139 |
| <I/σ(I)> | 8.92 | 29.7 | 1.6 |
| Completeness [%] | 99.2 | 86.3 | 95.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM dioxane, 9-11% PEG 6,000, 15mM DTT, vapor diffusion, temperature 293K, VAPOR DIFFUSION |
