3S1E
Pro427Gln mutant of maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979629 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 250.120, 50.110, 51.170 |
Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.1995 |
Rwork | 0.198 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bw7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.150 |
High resolution limit [Å] | 1.900 | 6.000 | 1.900 |
Rmerge | 0.060 | 0.026 | 0.363 |
Number of reflections | 48499 | ||
<I/σ(I)> | 15.66 | 41.79 | 4.17 |
Completeness [%] | 96.5 | 95.7 | 95.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% (w/v) PEG 1500, 0.5% (w/v)n-octyl beta-D-glucoside, 0.1 M Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |