3S1C
Maize cytokinin oxidase/dehydrogenase complexed with N6-isopentenyladenosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979629 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 250.950, 50.620, 51.400 |
Unit cell angles | 90.00, 93.97, 90.00 |
Refinement procedure
Resolution | 32.500 - 2.090 |
R-factor | 0.2148 |
Rwork | 0.213 |
R-free | 0.25240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bw7 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 2.140 |
High resolution limit [Å] | 2.090 | 8.800 | 2.090 |
Rmerge | 0.058 | 0.023 | 0.337 |
Number of reflections | 35133 | ||
<I/σ(I)> | 16.9 | 35.1 | 4.73 |
Completeness [%] | 91.1 | 90 | 97.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 30% (w/v) PEG 1500, 0.5% (w/v)n-octyl beta-D-glucoside, 0.1 M Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |