3S16

RNA Polymerase II Initiation Complex with an 8-nt RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-30
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	157.843, 221.125, 192.746
Unit cell angles	90.00, 97.61, 90.00

Refinement procedure

Resolution	29.865 - 3.241
R-factor	0.1855
Rwork	0.183
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nvq
RMSD bond length	0.009
RMSD bond angle	1.180
Data scaling software	SCALA
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.865	29.865	3.420
High resolution limit [Å]	3.241	10.250	3.240
Rmerge		0.052	0.811
Total number of observations		12248	55439
Number of reflections	103014
<I/σ(I)>	6.6	12.5	1
Completeness [%]	99.4	95.9	96.6
Redundancy	3.9	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM dioxane, 9-11% PEG 6.000, 15mM DTT, vapor diffusion, temperature 293K