3S15
RNA Polymerase II Initiation Complex with a 7-nt RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-30 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.928, 220.891, 194.623 |
| Unit cell angles | 90.00, 100.16, 90.00 |
Refinement procedure
| Resolution | 44.110 - 3.300 |
| R-factor | 0.1763 |
| Rwork | 0.174 |
| R-free | 0.22790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nvq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
| Rmerge | 0.132 | 0.035 | 0.761 |
| Number of reflections | 104957 | ||
| <I/σ(I)> | 8.9 | ||
| Completeness [%] | 99.9 | 99.7 | 99.9 |
| Redundancy | 3.8 | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM dioxane, 9-11% PEG 6.000, 15mM DTT, vapor diffusion, temperature 293K |
