3S14
RNA Polymerase II Initiation Complex with a 6-nt RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.335, 221.345, 192.368 |
| Unit cell angles | 90.00, 98.00, 90.00 |
Refinement procedure
| Resolution | 39.460 - 2.850 |
| R-factor | 0.1904 |
| Rwork | 0.189 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nvq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 6.140 | 2.850 |
| Rmerge | 0.110 | 0.033 | 0.911 |
| Number of reflections | 154876 | ||
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 99.9 | 99.8 | 99.8 |
| Redundancy | 3.8 | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM dioxane, 9-11% PEG 6,000, 15mM DTT, vapor diffusion, temperature 293K, VAPOR DIFFUSION |
