3S0Y
The crystal structure of the periplasmic domain of MotB (residues 64-256).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.004 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 75.210, 75.210, 124.707 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.720 - 1.800 |
R-factor | 0.1801 |
Rwork | 0.178 |
R-free | 0.22140 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.325 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.062 |
Number of reflections | 30716 |
<I/σ(I)> | 16.8 |
Completeness [%] | 91.0 |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.1 M ammonium sulphate, 100 mM MES monohydrate pH 6.5 and 2.5% v/v 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |