3S0W
The crystal structure of the periplasmic domain of Helicobacter pylori MotB (residues 78-256).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.704, 74.704, 126.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.1959 |
| Rwork | 0.193 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.453 |
| Data scaling software | SCALA (3.3.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.282 | 64.282 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.067 | 0.024 | 0.333 |
| Total number of observations | 2210 | 10248 | |
| Number of reflections | 12864 | ||
| <I/σ(I)> | 17.9 | 23.5 | 2.3 |
| Completeness [%] | 99.3 | 97.3 | 99 |
| Redundancy | 5.5 | 4.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM Tris pH 8.5, 2.5 M AS , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
