3S0J
The crystal structure of glycogen phosphorylase b in complex with 2,5-dihydroxy-4-(beta-D-glucopyranosyl)-chlorobenzene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2005-09-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8156 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.450, 128.450, 116.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.19105 |
| Rwork | 0.190 |
| R-free | 0.20947 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2prj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 64845 | |
| <I/σ(I)> | 21.2 | 5.2 |
| Completeness [%] | 93.9 | 90.9 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM BES Buffer, 3 mM DTT, 1 mM IMP, pH 6.7, SMALL TUBES, temperature 289K |
